법랑기질유도체가 탈회 치근표면에서 치주인대섬유아세포의 생물학적 성상에 미치는 영향
Effects of enamel matrix derivatives on biologic activities of human periodontal fibloblasts to demineralized root surface
이강운, 정종평, 한수부, 김태일, 설양조, 이용무, 구영, 류인철,
소속 상세정보
이강운 ( Lee Kang-Woon ) - 서울대학교 치의학대학원 치주과학교실
정종평 ( Chung Chong-Pyoung ) - 서울대학교 치의학대학원 치주과학교실
한수부 ( Han Soo-Boo ) - 서울대학교 치의학대학원 치주과학교실
김태일 ( Kim Tai-Il ) - 서울대학교 치의학대학원 치주과학교실
설양조 ( Seol Yang-Jo ) - 서울대학교 치의학대학원 치주과학교실
이용무 ( Lee Yong-Moo ) - 서울대학교 치의학대학원 치주과학교실
구영 ( Ku Young ) - 서울대학교 치의학대학원 치주과학교실
류인철 ( Rhyu In-Chul ) - 서울대학교 치의학대학원 치주과학교실
KMID : 0363020080380040679
Abstract
Purpose: The aim of this study was to investigate the effects of EMD on demineralized root surface using human periodontal ligament cells and compare the effects of root conditioning materials(tetracycline(TCN), EDTA).
Material and Methods: Dentin slices were prepared from the extracted teeth and demineralized with TCN and EDTA. Demineralized dentin slices were incubated at culture plate with 25, 50 and concentration of EMD. Cell attachment, alkaline phosphatase activity test, protein synthesis assay and scanning electronic microscopic examination were done.
Results: Cells were attached significantly higher in EMD treated group at 7 and 14 days. Cell numbers were significantly higher in EMD treated group. Alkaline phosphatase activity was significantly higher in EMD treated group at 7 and 14 days. Protein synthesis was significantly higher in EMD treated group at 7 and 14 days.
Conclusion: Enamel matrix derivatives enhance the biologic activities of human periodontal ligament cells on demineralized root surface and its effects are dependent on the concentration of EMD.
키워드
Enamel matrix derivatives;PDL cell;Tetracycline;EDTA
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